A simple, rapid and improved colorimetric assay for nontransferrin thalassemia patients bound iron estimation inthalassemia patients

Abstract

Iron in the plasma that is not attached to the protein transferrin, which normally carries it, is referred to as
"non transferrin bound iron." It has been hypothesized that the redox activity of such iron compounds, as
shown by Haiber weiss and Fenton chemistry, makes them poisonous. Non transferrin bound iron has been
the subject of much research in patients receiving blood transfusions for the treatment of iron overload
disorders such as hemochromatosis and thalassemia. Several academics have proposed studying NTBI to
evaluate the usefulness of chelators in treating iron excess. Different researchers have proposed different
waysfor measuring the amount of iron that is not bound to transferrin, such as chromatography, fluorimetry,
atomic absorption spectroscopy, spectrophotometry, etc. The considerable complexity of the process, the
need for expensive specialized equipment and accessories, and the questionable dependability mean that
none of them is still universally recognized or regarded gold standard. In order to make the
bathophenanthroline based colorimetric approach for non transferrin bound iron measurement accessible to
smaller labs with less resources without sacrificing accuracy, we have experimented with a number of
alternative variants. NTBI values were calculated for both -thalassemia patients and healthy controls using
the trial methodology that yielded the most stable findings. We discovered that the background noise was
much reduced and the most consistent findings were produced by high-speed centrifugation with interstitial
sample pre-incubation. There was a statistically significant difference between the means of the thalassemia
patient group and the control group for the quantity of non-transferrin bound iron.Hemochromatosis, beta
thalassemia, and bathophenanthroline are all diseases associated with an excess of iron that isn't bound to
transferrin.