Simultaneous Estimation of Antihypertensive Drugs Using Derivative Spectroscopy

Abstract

For the simultaneous measurement of atorvastatin calcium and amlodipine besylate, a straightforward, accurate, and precise UV spectroscopic approach has been established. The established approach of simultaneous equations is cost-effective and repeatable. 365 and 246 nm were the wavelengths used to measure the absorbance of the two medications, respectively. Amlodipine besylate and atorvastatin calcium showed linearity at their respective λmaxes of 365 and 246 nm as well as at the isoabsorptive point at 238 nm in a concentration range of 2 to 10 μg/mL. The accuracy of the approach was shown by recovery trials, which showed recovery of >99.78% for amlodipine besylate and >99.36% for atorvastatin calcium. The proposed approach is recommended for regular analysis due to its speed, ease of use, accuracy, sensitivity, and specificity. The International Council of Harmonization's (ICH) guidelines were followed for conducting the validation investigations.