Improving Levosulpiride and Rabeprazole's Bulk and Dosage Forms with the Use of High-Performance Liquid Chromatography and Its Validation

Abstract

The analytical technique is the bedrock of pharmaceutical analysis. A sensitive, easy-to-understand, and accurate method for concurrently
assessing levosulpiride (LEVO) and rabeprazole (RABE) was the objective of the present investigation. The mobile phase, which consisted
of acetonitrile and buffer at a ratio of 70:30, was more than enough. Our analysis found that LEVO could be recovered from 80%, 100%,
and 120% sample solutions with percentages of 99.98%, 100.06%, and 100.1%, respectively. Results showed that
RABE values of 98.99%, 99.46%, and 100.08% were obtained from the 80%, 100%, and 120% sample solutions, respectively. Both drugs'
percentage recoveries were within the expected range. This indicates that the proposed technique was more precise than the existing ones.
The method ensures accuracy by analyzing the sample enough to provide statistically significant results. Next, the precision is shown by
the percentage of the relative standard deviation. A relative standard deviation (RSD) of no more than 2% is considered acceptable for this
method's accuracy. Results showed that RABE had an intermediate accuracy percent RSD of 0.242 and LEVO had a value of 0.344,
according to the present study. The % RSD value indicates a high degree of accuracy within the provided range.