Development and Validation of UV Spectroscopic method for estimation of Ellagicacid in Herbal capsule used for the treatment of Diabetes

Abstract

With the use of ellagic acid estimate for Eugenia jamboloma's commercialized formulation and raw
material, a UV spectroscopic fingerprinting technique was created. For elagic acid, Beer Lambert's rule
holds true between 2 and 12 micrograms per milliliter at maximum absorbance of 280 nanometers. The
linearity between concentration and absorbance was evaluated using the correlation coefficient (r2), which
showed a value of 0.996. Formulated capsules (one commercial formulation) and Eugenia jamboloma each
had their ellagic acid content estimated independently. Eugenia jamboloma and MCM, a commercially
available formulation, were analyzed to assess their ellagic acid content. Spectrophotometric measurement
of ellagic acid using the current approach was found to be straightforward, precise, accurate, and amenable
to routine analysis of ellagic acid in chosen formulations based on the validation results.
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